- How are TLC plates measured?
- What causes Tailing in TLC?
- What are the uses of TLC?
- What do TLC results mean?
- How is TLC used in forensics?
- What are the limitations of TLC?
- Why silica gel is used in TLC?
- What is the principle of TLC?
- What happens if you apply too much compound on the TLC plate?
- Why should you use pencil instead of pen to mark a TLC plate?
- What do TLC plates tell you?
- Why should you not touch the TLC plate?
- How do you read TLC plate results?
How are TLC plates measured?
Handle the plates carefully so that you do not disturb the coating of adsorbent or get them dirty.
Measure 0.5 cm from the bottom of the plate.
Using a pencil, draw a line across the plate at the 0.5 cm mark.
This is the origin: the line on which you will spot the plate..
What causes Tailing in TLC?
Compounds which is basic in nature are often tailing on silica coated TLC plate because silica is acidic in nature so they interact with one another and doing tailing. … Some time if we load maximum compound on TLC plate, in this condition we facing solubility problem. In this problem tailing also occur.
What are the uses of TLC?
TLC is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance.
What do TLC results mean?
Thin layer chromatographyThin layer chromatography, or TLC, is a method for analyzing mixtures by separating the compounds in the mixture. TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound.
How is TLC used in forensics?
Separation of complex mixtures (known as chromatography) is an essential tool in forensic science. It is routinely used to identify and compare samples of drugs, explosives, inks and biological samples such as saliva, urine, blood and other.
What are the limitations of TLC?
Disadvantages of TLC include application to only nonvolatile compounds, limited resolution capability (separation numbers or peak capacities of 10–50), and the absence of fully automated systems, although the individual steps of the technique can be automated.
Why silica gel is used in TLC?
Silica gel is by far the most widely used adsorbent and remains the dominant stationary phase for TLC. … The surface of silica gel with the highest concentration of geminal and associated silanols is favored most for the chromatography of basic compounds because these silanols are less acidic.
What is the principle of TLC?
Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).
What happens if you apply too much compound on the TLC plate?
Answer: The ink might travel with the eluting solvent and separate into its component pigments, giving you a lot of extraneous spots. 3) What could happen if you spot too much of a compound on the TLC plate? Answer: The spot would show trailing. … Answer: The spots would dissolve into the reservoir of eluting solvent.
Why should you use pencil instead of pen to mark a TLC plate?
Pencil is always used to mark chromatography paper or TLC plates because ink may run and interfere with the chromatogram. … As soon as the paper/plate is taken out, mark the solvent front with a pencil before the solvent evaporates and the front becomes impossible to see.
What do TLC plates tell you?
Thin Layer Chromatography (TLC) is a separation technique requiring very little sample. It is primarily used to determine the purity of a compound. A pure solid will show only one spot on a developed TLC plate. In addition, tentative identification of the unknown compound can be made through TLC analysis.
Why should you not touch the TLC plate?
Never under any circumstances touch the face of a TLC plate with your fingers as contamination from skin oils or residues on gloves can obscure results. Instead, always handle them by the edges, or with forceps. The properties of your sample should be considered when selecting the stationary phase.
How do you read TLC plate results?
In simple terms, this value is an indication of how far up a TLC-plate a compound has wandered. A high Rf -value indicates that the compound has travelled far up the plate and is less polar, while a lower Rf -value indicates that the compound has not travelled far, and is more polar.